PTSA

the Pig Testis Spatial Atlas

Pig testicular development spatiotemporal atlas database, integrating single-cell transcriptome and spatial transcriptome data | 13 developmental stages spanning from F60 to 50 months.

What Can This Database Do for You?

Developmental Trajectory Tracking – Explore pig testis development from F60 to 50 months
Cell Atlas Analysis – Inspect cell types and gene expression dynamics
Gene Expression Dynamics – Query expression across stages and cell types
UMAP Visualization – Browse gene expression on UMAP embeddings
Cross-Stage Comparison – Compare cellular composition and expression across periods

Single-Cell Analysis and Spatial Transcriptomics

Search by cell type Search by gene Search by UMAP All stages

Available demo genes

SOX9 DDX4 AMH SPATA3 DNAAF11 CYP11A1 INHA STAR CLU ACTA2

Developmental stages

Integrated analysis of spatially resolved multi-omics data across the pig testicular developmental timeline.

We selected 13 developmental time points spanning fetal stages to reproductive ageing stages for atlas construction: fetal stages (F60 and F90), neonatal stage (3d), pre-weaning stage (30d), nursery stage (60d), pre-pubertal stage (90d), puberty (130d, 160d), young adulthood stage (10m), adulthood stage (24m), Middle-aged stage (35m) and reproductive ageing stages (46m, 50m), selected based on physiological milestones along with practical considerations in swine breeding.

F60 F90 3d 30d 60d 90d 130d 160d 10m 24m 35m 46m 50m ERC

UMAP visualization of testicular cells profiled by pig scRNA-seq data

UMAP of pig testicular cells derived from scRNA-seq and Stereo-seq across developmental stages.

Cell types

Spermatogonial stem cells (SSC) Spermatogonias (SPG) Early primary spermatocytes (E_SPC) Late primary spermatocytes (L_SPC) Round Spermatids (R_SPT) Elongating and elongated spermatids (E_SPT) Progenitor Leydig cells (P_Leydig) Leydig cells (Leydig) Progenitor Sertoli cells (P_Sertoli) Sertoli cells (Sertoli) Progenitor Peritubular myoid cells (P_PMC) Peritubular myoid cells (PMC) Vascular smooth muscle cells (SMC) Vascular endothelial cells (EC) B cells T cells Macrophages (Mph)

Publication

Siwen Wu¹, Pinli Hu¹, Zhili Li¹, Di Zhu¹, Qianxi Liang, Qing Lin, Xing Yang, Xiaoxian Xu, Chaobin Qin, Junchao Wang, Meiqin Cai, Renqiang Yuan, Xuehua Li, Zilu Zhang, Dongze Li, Panjie Xia, Mingqiong Li, Lin Zhu, Hong Lan, Xiaolong Lu, Jing Liu, Jiuju Luo, Haiyong Zhou, Zhanwei Zhuang, Fumei Chen, Zhe Zhang, Wansheng Liu, Longqi Liu, Shuhong Zhao, Qingyou Liu^2,*, Haojing Shao^2,*, Lingzhao Fang^2,*, Yunxiang Zhao^2,*, A spatiotemporal multi-omic atlas of the pig testis decodes the genetic and molecular architecture underlying mammalian spermatogenesis and male fertility (under review).